Frequently Asked Questions
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Selected Frequently Asked Questions
How do you prepare your cryopreserved hepatocytes?
The liver tissue is perfused and the cells are isolated. Percoll® centrifugation is performed to improve the cell viability. Finally, the cells are cryopreserved as described in our manuscript: "Effect of cryopreservation on cytochrome P450 enzyme induction in cultured rat hepatocytes," A Madan, R DeHaan, D Mudra, K Carroll, E LeCluyse and A Parkinson, Drug Metab Dispos, 27:327-335, 1999.
How are cryopreserved hepatocytes shipped?
The hepatocytes are shipped in a "dry dewar." This is a cryogenic shipper containing vapor phase liquid nitrogen. The containers include a return airbill for easy return to XenoTech.
Can I order pooled human hepatocytes?
XenoTech offers individual human hepatocytes in a “Pooling Category.” Each vial has an assured minimum viable yield of 1.0 x 106 cells based on post-thaw data. This provides a cost-effective method for purchasing several different individuals human vials, which can be pooled upon thawing.