time-dependent inhibiton
This is a general term used to refer to any increase in inhibitory potential that occurs as an enzyme is exposed to an inhibitor over time, and does not necessarily require a metabolic step. Time-dependent inhibition may include the following:
- slow-binding inhibition
- inhibition by a degradation product (i.e., non-enzymatic conversion to an inhibitory species
- reversible metabolism-dependent inhibition
- irreversible metabolism-dependent inhibition
Trypan Blue
Large molecular weight cellular dye, which is taken up exclusively by cells with compromised membranes (non-viable cells). It is used in determining viability of a cell preparation. Due to its size, the dye cannot penetrate and will not color live cells.