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Cryopreserved Beagle Dog Hepatocytes

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DESCRIPTION
CHARACTERIZATION PROVIDED
RELATED PRODUCTS
PRODUCTS

DESCRIPTION


Upon thawing, our pooled cryopreserved Beagle dog hepatocytes remain viable (data shown below) and may be used in suspension for short-term drug metabolism experiments (we recommend incubations no longer than four hours). When cryopreserved Beagle dog hepatocytes are thawed, isolated and suspended under appropriate conditions, they can be used to evaluate the in vitro metabolism of drug candidates.

Please note that XenoTech does not recommend cryopreserved dog hepatocytes for use in culture because their attachment to cell culture dishes is unpredictable.

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CHARACTERIZATION PROVIDED


Characterization data from a representative lot of D1000.H15

Enzyme

Marker Substrate Reaction

pmol/cells per inc/min

Phase I

7-Ethoxycoumarin O-dealkylation

251

Phase II

7-Hydroxycoumarin glucuronidation

70.7

Phase II

7-Hydroxycoumarin sulfation

78.8


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PRODUCTS


Product No.

Product

Gender

Assured Minimum Yield*

Amount

D1000.H15

Cryopreserved Beagle Dog Hepatocytes

Male

3.5x106 cells

1.5 mL

D1000.H45

Cryopreserved Beagle Dog Hepatocytes

Male

12x106 cells

4.5 mL

D1500.H15

Cryopreserved Beagle Dog Hepatocytes

Female

3.5x106 cells

1.5 mL
* AMY is based on experiments performed at XenoTech using XenoTech's thawing protocol (PDF) and Hepatocyte Isolation Kit.

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