Cryopreserved Human Hepatocytes for Suspension
View ProductsAvailable Human Hepatocyte Lot Characterization (PDF)PACKAGINGCHARACTERIZATION PROVIDEDRELATED PRODUCTSSAFETY, HANDLING AND STORAGEPRODUCTSPACKAGING
XenoTech's cryopreserved human hepatocytes are available in Assured Minimum Yield (AMY) Categories, so you can choose the number of viable cells that you need for your experiment.
Category |
Assured Minimum Yield per Vial* |
1 |
1.0x106 cells |
2 |
2.0x106 cells |
3 |
3.0x106 cells |
4 |
4.0x106 cells |
6 |
6.0x106 cells |
*Vial size is 1.5 mLReturn to Top
CHARACTERIZATION PROVIDED
Available Human Hepatocyte Lot Characterization (PDF)
- % Viability
- Phenacetin O-dealkylation
- Coumarin 7-hydroxylation
- Bupropion hydroxylation
- Amodiaquine N-dealkylation
- Diclofenac 4'-hydroxylation
- S-Mephenytoin 4'-hydroxylation
- Dextromethorphan O-demethylation
- Chlorzoxazone 6-hydroxylation
- Testosterone 6β-hydroxylation
- Midazolam 1'-hydroxylation
- Lauric acid 12-hydroxylation
Return to TopSome experiments require pooled samples, but the logistics of human tissue receipt and the sensitivity of cells to multiple cryopreservation cycles make this an interesting challenge when using human hepatocytes.
XenoTech has devised a kit that allows you to choose cells from three donors (n=3) and conveniently pool them in a single thaw/isolation step.*
Pooling made easy: Each kit includes 3 vials of cryopreserved human hepatocytes (your choice of lots from Category 1 or 2) for a savings of 25% off the regular list price.
Product No. |
Product |
Amount |
K3001 |
Pooled human hepatocytes kit, 3 vials from category 1 |
3 million cells/kit |
K3002 |
Pooled human hepatocytes kit, 3 vials from category 2 |
6 million cells/kit |
*We recommend using our Hepatocyte Isolation Kit (Product ID K2000), sold separately.
Return to Top
SAFETY, HANDLING AND STORAGE
Informed Consent Statement
A single organization regulates and oversees the use of human tissue intended for transplantation in the United States, namely the United Network for Organ Sharing (UNOS). Organ donors may elect to have their organs used either for transplantation only, or for transplantation or research. Thus, the donor (or the donor's family) has the right to prevent the use of the donor's organs for research. Regardless of the use of donated organs, no compensation is given to the donor’s family; any such compensation is illegal in the United States. In those cases where donors (or family members) elect to withhold organs from research uses, any organs that cannot be transplanted are discarded.
XenoTech receives hepatic, renal, intestinal, pulmonary, and other human tissue from various regional organ procurement organizations (OPOs) that obtain organs approved for research use. Regulations in the United States require that, regardless whether the organ is intended for transplantation or research purposes, the organ donor's identity be treated as highly confidential information. Organ procurement organizations maintain the informed consent records from each donor, and our Standard Operating Procedure requires that XenoTech personnel confirm the existence of informed consent for research purposes, prior to transport of organs to XenoTech. This procedure is intended to ensure that XenoTech manufactures human-derived products only when informed consent has been granted for research use of those specific organs. XenoTech does not, and, in consideration of confidentiality, cannot obtain the informed consent records from these organizations.
All human tissue accepted by XenoTech has been tested for the possible presence of various infectious diseases, and XenoTech does not accept human-derived material unless the donor has tested negative (non-reactive) for RPR, HIVAb, HTLVAb, HBsAg and HCVAb. All human tissue is also tested for CMVAb. However, due to the widespread (nearly ubiquitous) appearance of CMV in the population, and its relative insignificance as an infectious agent, tissues from donors reactive for CMVAb are accepted. The serology status of each donor is typically determined by ELISA by the organ procurement hospital.
XenoTech does not deal directly with - nor does it make any direct payments to - the surgeons who procure organs or the medical institutions where they work.
Cryopreserved hepatocytes should be stored in liquid nitrogen vapor (-160°C or colder). They should be thawed in accordance with our
thawing protocol (PDF), which is shipped with your hepatocytes.
Upon thawing, cryopreserved human hepatocytes remain viable and may be used in suspension for short-term drug
metabolism experiments (we recommend incubations no longer than 4 hours). When cryopreserved human hepatocytes are thawed, isolated, and suspended under appropriate conditions, they can be used to evaluate the metabolism of drug candidates.
Caution: In the case of human tissue, XenoTech accepts only non-transplantable tissue from donors who test negative for HIV 1 and 2, HTLV, and Hepatitis B and C. However, as a precaution, all human-derived samples should be regarded as a potential biohazard and should be stored, handled and discarded accordingly. Cellular and subcellular fractions are intended for in vitro use only.
Return to TopPRODUCTS
Product No. |
Product |
Gender |
Assured Minimum Yield* |
H1000.H15P |
Cryopreserved Human Hepatocytes for Suspension |
Male |
1.0 million cells |
H1000.H15A |
Cryopreserved Human Hepatocytes for Suspension |
Male |
2.0 million cells |
H1000.H15-3 |
Cryopreserved Human Hepatocytes for Suspension |
Male |
3.0 million cells |
H1000.H15B |
Cryopreserved Human Hepatocytes for Suspension |
Male |
4.0 million cells |
H1000.H15C |
Cryopreserved Human Hepatocytes for Suspension |
Male |
6.0 million cells |
H1500.H15P |
Cryopreserved Human Hepatocytes for Suspension |
Female |
1.0 million cells |
H1500.H15A |
Cryopreserved Human Hepatocytes for Suspension |
Female |
2.0 million cells |
H1500.H15-3 |
Cryopreserved Human Hepatocytes for Suspension |
Female |
3.0 million cells |
H1500.H15B |
Cryopreserved Human Hepatocytes for Suspension |
Female |
4.0 million cells |
H1500.H15C |
Cryopreserved Human Hepatocytes for Suspension |
Female |
6.0 million cells |
* AMY is based on experiments performed at XenoTech using XenoTech's
thawing protocol (PDF) and
Hepatocyte Isolation Kit.
Return to TopOrder Fax: 913.227.7171
Order E-mail: info@xenotechllc.com
Call 877.588.7530 (toll-free US and Canada) for pricing and availability.
If ordering outside of North America, please see our Distributor information.