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B6C3F1 Mouse Subcellular Fractions

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XenoTech offers pooled liver microsomes, S9 fractions and cytosol from sexually-mature male B6C3F1 mice.

DESCRIPTION
PACKAGING
CHARACTERIZATION PROVIDED
PRODUCT DETAILS
PRODUCTS

DESCRIPTION


Liver microsomes con­tain cytochromes P450, flavin-monooxygenases, carboxyl­esterases, epoxide hydrolase, UDP-glu­cur­ono­syl­transferases and other drug-metabolizing enzymes, and are intended for use in in vitro studies of xenobiotic metabolism.

The S9 fraction is a mixture of microsomes and cytosol. Accordingly, it contains a wide variety of Phase 1 and Phase 2 enzymes including P450 enzymes, flavin-mono­oxygenases, carboxyl­esterases, epoxide hydrolase, UDP-glucuronosyl­transferases, sulfotransferases, methyl­transferases, acetyl­trans­ferases, gluta­thione S‑trans­fer­ases and other drug-metabolizing enzymes.

Liver cytosol contains many xenobiotic metabolizing enzymes including carboxylesterases, soluble epoxide hydrolases, alcohol dehydrogenases, aldehydes oxidases, xanthine oxidases, diamine oxidases, sulfotransferases, glutathione S‑transferases, etc.

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PACKAGING


Micro­somes
Concentration: 20 mg/mL
Suspension buffer: 250 mM sucrose

S9 fraction
Concentration: 20 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

Cytosol
Concentration: 10 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA 

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CHARACTERIZATION PROVIDED


The following lot-specific product information is included with each order of non-human liver microsomes.
  • Specific content of cytochrome P450
  • Specific content of cytochrome b5
  • NADPH-cytochrome c reductase activity
  • 7-Ethoxycoumarin O-dealkylation
  • Animal Information and Husbandry
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PRODUCT DETAILS


NOMENCLATURE B6C3F1/CrlBR ORIGIN A cross between female C57BL/6NCrlBR x male C3H/HeNCrlBR. COAT COLOR Agouti (wild type).

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PRODUCTS


Product No.

Product

Gender

Amount

M2000

Pooled B6C3F1 Mouse Liver Microsomes

Male

0.5 mL at 20 mg/mL

M2000.S9

Pooled B6C3F1 Mouse Liver S9 Fraction

Male

1.0 mL at 20 mg/mL

M2000.C

Pooled B6C3F1 Mouse Liver Cytosol

Male

1.0 mL at 10 mg/mL


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Order Fax: 913.227.7171
Order E-mail: info@xenotechllc.com
Call 877.588.7530 (toll-free US and Canada) for pricing and availability.
If ordering outside of North America, please see our Distributor information.