Cynomolgus Monkey Kidney Subcellular Fractions
View Products DESCRIPTIONPACKAGINGCHARACTERIZATION PROVIDEDRELATED PRODUCTSSAFETY, HANDLING AND STORAGEPRODUCTSDESCRIPTION
Drug-metabolizing enzymes in the kidney play a significant role in the metabolism of many xenobiotics. Kidneys have many metabolic responsibilities including the hydroxylation of fatty acids and their derivatives, which is one of the functions of the CYP4A and CYP4B enzymes expressed in renal microsomes. There is increasing interest in renal metabolism due to the significant role that kidneys play in the metabolism of drugs and the fact that the kidney is a major site of drug-induced toxicity.
To assess the stability of human and animal renal subcellular fractions, pooled samples were subjected to several freeze-thaw cycles, and analyzed for lauric acid 12-hydroxylation, umbelliferone glucuronidation and NADPH-cytochrome
c reduction. As shown below, renal samples prepared by XenoTech’s subcellular fractionation protocol can be repeatedly frozen and thawed at least ten times without significant loss of CYP4A activity. Similar results were obtained for umbelliferone glucuronidation and NADPH-cytochrome
c reduction.
Return to TopPACKAGING
MicrosomesConcentration: 10 mg/mL
Suspension buffer: 250 mM sucrose
S9 fraction
Concentration: 5 mg/mL
Suspension buffer: 50 mM TRIS
·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA
Return to TopCHARACTERIZATION PROVIDED
- NADPH-cytochrome c reductase
- Lauric Acid 12-hydroxylation
- 4-Methylumbelliferone glucuronidation
Return to TopXenoTech also offers subcellular fractions of kidney tissue from the following toxicologically significant species:
For other extrahepatic or subcellular fractions, please
contact us to discuss our wide selection of
Custom Products. We have extensive experience preparing a variety of unique research products (from human and laboratory animal sources) for a growing list of customers.
Return to TopSAFETY, HANDLING AND STORAGE
Policy statement concerning the safety of non-human primate-derived material
XenoTech manufactures products derived from non-human primate tissue for the purpose of xenobiotic research.
All tissue used to manufacture these products is obtained from reliable sources within the
United States, and is obtained from disease-free (asymptomatic) animals under the care of a veterinarian.
In accordance with federal (USDA) regulations, all non-human primates entering the
United States are placed in quarantine for a period of thirty days.
If an animal is infected with Ebola virus, it would die during the quarantine period, and hence, would not reside in a primate colony in the
United States.
For this reason, Ebola virus testing is not a common procedure in the
United States.
Animals are tested for Simian Herpes B virus (SBV), Simian Retrovirus (SRV), and Simian Immunodeficiency virus (SIV).
All non-human primate tissue utilized at XenoTech is obtained from animals which have tested seronegative for these viruses.Although animals are quarantined and tested for certain infectious diseases, it is not scientifically possible to guarantee that non-human primate-derived material is free of
all infectious agents and poses absolutely no health risk.
Therefore, we strongly caution all researchers who use non-human primate-derived material to treat such material as a potential biohazard, and to observe all national, regional, and local regulations governing the handling of such material.
Even if non-human primate-derived material has been tested for the presence of specific infectious agents by highly sensitive techniques, such as RT-PCR, and found to test negative, the material should still be treated as a potential biohazard.
PRODUCTS
Product No. |
Product |
Gender |
Amount |
P2000.R |
Pooled Cynomolgus Monkey Renal Microsomes |
Male |
0.5 mL at 10 mg/mL |
P2000.RS9 |
Pooled Cynomolgus Monkey Renal S9 Fraction |
Male |
1.0 mL at 5 mg/mL |
Return to TopOrder Fax: 913.227.7171
Order E-mail: info@xenotechllc.com
Call 877.588.7530 (toll-free US and Canada) for pricing and availability.
If ordering outside of North America, please see our Distributor information.