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Fischer 344 Rat Subcellular Fractions

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XenoTech offers pooled liver microsomes, S9 fractions and cytosol from sexually-mature male and female Fischer 344 rats.

DESCRIPTION
PACKAGING
CHARACTERIZATION PROVIDED
PRODUCT DETAILS
PRODUCTS

DESCRIPTION


Liver microsomes con­tain cytochromes P450, flavin-monooxygenases, carboxyl­esterases, epoxide hydrolase, UDP-glu­cur­ono­syl­transferases and other drug-metabolizing enzymes, and are intended for use in in vitro studies of xenobiotic metabolism.

The S9 fraction is a mixture of microsomes and cytosol. Accordingly, it contains a wide variety of Phase 1 and Phase 2 enzymes including P450 enzymes, flavin-mono­oxygenases, carboxyl­esterases, epoxide hydrolase, UDP-glucuronosyl­transferases, sulfotransferases, methyl­transferases, acetyl­trans­ferases, gluta­thione S‑trans­fer­ases and other drug-metabolizing enzymes.

Liver cytosol contains many xenobiotic metabolizing enzymes including carboxylesterases, soluble epoxide hydrolases, alcohol dehydrogenases, aldehydes oxidases, xanthine oxidases, diamine oxidases, sulfotransferases, glutathione S‑transferases, etc.

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PACKAGING


Micro­somes
Concentration: 20 mg/mL
Suspension buffer: 250 mM sucrose

S9 fraction
Concentration: 20 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

Cytosol
Concentration: 10 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

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CHARACTERIZATION PROVIDED


The following lot-specific product information is included with each order of non-human liver microsomes.
  • Specific content of cytochrome P450
  • Specific content of cytochrome b5
  • NADPH-cytochrome c reductase activity
  • 7-Ethoxycoumarin O-dealkylation
  • Animal Information and Husbandry
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PRODUCT DETAILS


NOMENCLATURE F344/CrCrlBR ORIGIN Curtis and Dunning in 1920 at Columbia University Institute for Cancer Research. To Heston in 1949. To National Institutes of Health in 1951. To CRL in 1998. COAT COLOR Albino.

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PRODUCTS


Product No.

Product

Gender

Amount

R2000

Pooled Fischer 344 Rat Liver Microsomes

Male

0.5 mL at 20 mg/mL

R2000.S9

Pooled Fischer 344 Rat Liver S9 Fraction

Male

1.0 mL at 20 mg/mL

R2000.C

Pooled Fischer 344 Rat Liver Cytosol

Male

1.0 mL at 10 mg/mL

R2500

Pooled Fischer 344 Rat Liver Microsomes

Female

0.5 mL at 20 mg/mL

R2500.S9

Pooled Fischer 344 Rat Liver S9 Fraction

Female

1.0 mL at 20 mg/mL

R2500.C

Pooled Fischer 344 Rat Liver Cytosol

Female

1.0 mL at 10 mg/mL


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Order Fax: 913.227.7171
Order E-mail: info@xenotechllc.com
Call 877.588.7530 (toll-free US and Canada) for pricing and availability.
If ordering outside of North America, please see our Distributor information.