Wistar Rat Subcellular Fractions
View Products XenoTech offers pooled liver
microsomes,
S9 fractions and
cytosol from sexually-mature male and female Wistar rats.
DESCRIPTIONPACKAGINGCHARACTERIZATION PROVIDEDPRODUCT DETAILSPRODUCTSDESCRIPTION
Liver microsomes contain cytochromes P450, flavin-monooxygenases, carboxylesterases, epoxide hydrolase, UDP-glucuronosyltransferases and other drug-metabolizing enzymes, and are intended for use in
in vitro studies of xenobiotic metabolism.
The
S9 fraction is a mixture of microsomes and cytosol. Accordingly, it contains a wide variety of Phase 1 and Phase 2 enzymes including P450 enzymes, flavin-monooxygenases, carboxylesterases, epoxide hydrolase, UDP-glucuronosyltransferases, sulfotransferases, methyltransferases, acetyltransferases, glutathione
S‑transferases and other drug-metabolizing enzymes.
Liver cytosol contains many xenobiotic metabolizing enzymes including carboxylesterases, soluble epoxide hydrolases, alcohol dehydrogenases, aldehydes oxidases, xanthine oxidases, diamine oxidases, sulfotransferases, glutathione
S‑transferases, etc.
Return to topPACKAGING
MicrosomesConcentration: 20 mg/mL
Suspension buffer: 250 mM sucrose
S9 fractionConcentration: 20 mg/mL
Suspension buffer: 50 mM TRIS
·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA
CytosolConcentration: 10 mg/mL
Suspension buffer: 50 mM TRIS
·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA
Return to topCHARACTERIZATION PROVIDED
The following lot-specific product information is included with each order of non-human liver microsomes.
- Specific content of cytochrome P450
- Specific content of cytochrome b5
- NADPH-cytochrome c reductase activity
- 7-Ethoxycoumarin O-dealkylation
- Animal Information and Husbandry
Return to topPRODUCT DETAILS
NOMENCLATURE Crl:(WI)BR
ORIGIN To Scientific Products Farm, Ltd. [predecessor of Charles River United Kingdom (CRUK)] in 1947 from Wistar Institute. To CRL in 1975 from CRUK. Caesarean rederived in 1975 to form the nucleus of the current Wistar rat colonies. This particular colony was selected because of a low incidence of hydronephrosis.
COAT COLOR Albino.
Return to topPRODUCTS
Product No. |
Product |
Gender |
Amount |
R3000 |
Pooled Wistar Rat Liver Microsomes |
Male |
0.5 mL at 20 mg/mL |
R3000.S9 |
Pooled Wistar Rat Liver S9 Fraction |
Male |
1.0 mL at 20 mg/mL |
R3000.C |
Pooled Wistar Rat Liver Cytosol |
Male |
1.0 mL at 10 mg/mL |
R3500 |
Pooled Wistar Rat Liver Microsomes |
Female |
0.5 mL at 20 mg/mL |
R3500.S9 |
Pooled Wistar Rat Liver S9 Fraction |
Female |
1.0 mL at 20 mg/mL |
R3500.C |
Pooled Wistar Rat Liver Cytosol |
Female |
1.0 mL at 10 mg/mL |
Return to TopOrder Fax: 913.227.7171
Order E-mail: info@xenotechllc.com
Call 877.588.7530 (toll-free US and Canada) for pricing and availability.
If ordering outside of North America, please see our Distributor information.