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Calendar of Events
| Date | Event Name |
|---|---|
| Sep 4 - 8, 2010 | ISSX EU Meeting |
| Sep 13 - 17, 2010 | Land O'Lakes Drug Metabolism |
| Nov 14 - 18, 2010 | AAPS 2010 |
Cryopreserved Human Hepatocytes For Induction
PackagingCharacterization Provided
XenoTech routinely checks each lot of cyropreserved human hepatocytes to ascertain their ability to attach to collagen substratum and to respond to the prototypical inducers, omeprazole and rifampin. Each lot that meets our performance criteria is characterized to determine fold induction of CYP1A2 and 3A4.
A photomicrograph of the plated cells, taken prior to treatment, is featured on each datasheet; an example from a representative lot is shown here.
Packaging
XenoTech's cryopreserved human hepatocytes are available in Assured Minimum Yield (AMY) Categories, so you can choose the number of viable cells that you need for your experiment.
| Category | Assured Minimum Yield per Vial* |
| P+ | 1.0x10^6 cells |
| A+ | 2.0x10^6 cells |
| 3+ | 3.0x10^6 cells |
| B+ | 4.0x10^6 cells |
| C+ | 6.0x10^6 cells |
*Vial size is 1.5 mL
Characterization Provided
Induction data from a representative lot of H1000.H15+
| Enzyme | Prototypical Inducer | Marker Substrate Reaction | Fold Induction* |
| CYP1A2 | Omeprazole (100 µM) | Phenacetin O-deethylation | 92.3 |
| CYP2B6 | Phenobarbital (750µM) | Bupropion hydroxylation | 10.9 |
| CYP3A4 | Rifampin (10 µM) | Testosterone 6b-hydroxylation |
4.94 |
* Refers to the increase in pmol metabolite formed when compared to control.
We recommend the use of supplemented Modified Chee’s Medium (K2300) with all cultured hepatocytes for overlay, feeding and dosing.
Additional characterization provided:
• % Viability
• Phenacetin O-dealkylation
• Coumarin 7-hydroxylation
• Bupropion hydroxylation
• Amodiaquine N-dealkylation
• Diclofenac 4'-hydroxylation
• S-Mephenytoin 4'-hydroxylation
• Dextromethorphan O-demethylation
• Chlorzoxazone 6-hydroxylation
• Testosterone 6β-hydroxylation
• Midazolam 1'-hydroxylation
• Lauric acid 12-hydroxylation
• 7-Hydroxycoumarin glucuronidation
• 7-Hydroxycoumarin sulfonation
Products
| Product # | Name | Gender | Assured Minimum Yield |
| H1000.H15-3+ | Cryopreserved Human Hepatocytes for Induction | Male | 3.0 million cells |
| H1000.H15A+ | Cryopreserved Human Hepatocytes for Induction | Male | 2.0 million cells |
| H1000.H15B+ | Cryopreserved Human Hepatocytes for Induction | Male | 4.0 million cells |
| H1000.H15C+ | Cryopreserved Human Hepatocytes for Induction | Male | 6.0 million cells |
| H1000.H15P+ | Cryopreserved Human Hepatocytes for Induction | Male | 1.0 million cells |
| H1500.H15-3+ | Cryopreserved Human Hepatocytes for Induction | Female | 3.0 million cells |
| H1500.H15A+ | Cryopreserved Human Hepatocytes for Induction | Female | 2.0 million cells |
| H1500.H15B+ | Cryopreserved Human Hepatocytes for Induction | Female | 4.0 million cells |
| H1500.H15C+ | Cryopreserved Human Hepatocytes for Induction | Female | 6.0 million cells |
| H1500.H15P+ | Cryopreserved Human Hepatocytes for Induction | Female | 1.0 million cells |
