Fa2N-4 Immortalized Human Hepatocytes

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The immortalized hepatocyte cell line (designated Fa2N-4) was isolated from normal human hepatocytes transformed with SV40 virus large T-antigen. The cell line is homozygous for the wild type alleles (*1/*1) of CYP2D6, CYP2C9 and CYP2C19, three cytochrome P450 enzymes that show a high degree of genetic polymorphism. Fa2N-4 hepatocytes attach to collagen and adopt the well-defined, cuboidal shape that is characteristic of normal hepatocytes. The morphology of these immortalized cells in culture (below right) closely resembles that of primary cultures of human hepatocytes (below left).

Packaging

Immortalized Human Hepatocytes are available in the following formats:

• 1.5 mL cryopreserved vials

• Freshly plated on 6, 12, 24 or 96 well plates

These products are intended for one-time use only. A shrink wrap agreement will be provided with each shipment and by breaking the seal on the package you are agreeing to the terms of the agreement.

Characterization Provided

*Refers to the increase in pmol metabolite formed when compared to control

We recommend using MFE Essential Plating, Support and Culture Medium Supplement A to obtain the best results with the Fa2N-4 cell line.

Safety, Handling, and Storage

Cryopreserved immortalized human hepatocytes should be stored in liquid nitrogen vapor (-160°C or colder). They should be thawed in accordance with our thawing and plating protocol (PDF), which is shipped with your hepatocytes.

Plated immortalized human hepatocytes should be stored in an incubator at 37°C, 95% humidity, 5% CO2 until ready to replace media. Please follow our plating protocol (PDF), which is shipped with your hepatocytes.

Related Products

• MFE Media
• Fresh human hepatocytes
• Cryopreserved inducing human hepatocytes

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