Reaction Phenotyping Kit

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Safety, Handling, and Storage

(U.S. Patent #5,478,723)

XenoTech's patented Reaction Phenotyping Kit (RPK) is designed to identify the human liver CYP or UGT enzyme(s) responsible for metabolizing a drug (or other xenobiotic), in order to predict pharmacokinetic variability, which can occur when a drug is metabolized by a polymorphically-expressed CYP or UGT enzyme. Reaction phenotyping (enzyme mapping) also provides valuable information on the potential for drug-drug interactions. Samples in the kit are carefully selected to minimize correlations or outliers that can interfere with reliable results.

Safety, Handling, and Storage

Storage

The Reaction Phenotyping Kit should be stored in an ultra-low freezer (-70°C or colder). At ultra-low temperatures, XenoTech’s human liver microsomes are stable for several years, as reported by Pearce et al.

R. Pearce, C.J. McIntyre, A. Madan, U.Sanzgiri, A.J. Draper, P. Bullock, D.C. Cook, L.A. Burton, J. Latham, C. Nevins, and A. Parkinson. Effects of freezing, thawing, and storing human liver microsomes on cytochrome P450. Arch. Biochem. Biophys. 331, 145-169, 1996.

Freezing and thawing samples

XenoTech’s human liver microsomes can be frozen and thawed as many as ten times with no apparent loss of P450 activity, as reported by Pearce et al. (cited above). Consequently, after an aliquot of human liver microsomes is taken from a vial, the residual sample can be re-frozen and used at a later date. Care should be taken to keep thawed samples on ice (-4°C) and to return them as quickly as possible to an ultra-low freezer for storage.

Incubation Conditions

For drug metabolism studies, liver microsomes can be incubated with a drug under a variety of conditions; therefore, the experimental conditions described here are provided simply as a guide. Suggested Incubation Conditions

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