Hepatocyte Isolation Kit

Now optimized for different species, our Hepatocyte Isolation Kits make it convenient to achieve the best possible preparations of hepatocytes. Each one-time-use kit is sufficient to thaw and isolate one 4.5-mL vial of hepatocytes, or up to three 1.5-mL vials. For best results, we have developed a separate kit for use with mouse hepatocytes that maintains a high post-thaw percent viability.

Download the XenoTech media flyer here!

Tube A contains nutrient-richmedia with an isotonic PercollTM used in initial cell isolation.  PercollTM from sigma Chemical Co., St. Louis, MO

Tube B contains supplemented nutrient-rich media used to wash the isolated hepatocytes

Counting Tube contains Trypan Blue solution used to determine cell yield and viability counts

Our non-gradient hepatocyte thawing kit contains supplemented nutrient-rich media used to wash the isolated hepatocytes and counting tubes containing trypan blue solution used to determine cell yield and viability counts. 

CLICK HERE to watch the hepatocyte thawing protocol video!

Products

Product #NameAmountDescription
K2000Hepatocyte Isolation KitKitPercoll™ solution and supplemented media to isolate and wash cryopreserved cells, plus Trypan blue solution to obtain accurate cell yield and viability counts.
K2100Rodent Hepatocyte Isolation KitKitSimilar to K2000, but specially formulated to give the optimal yield and percent viability when used with rodent hepatocytes.
K2400Non-Gradient Hepatocyte Thawing KitKitSupplemented media to isolate and wash cryopreserved cells, plus Trypan blue solution to obtain accurate cell yield and viability counts.