Beagle Dog Kidney Subcellular Fractions

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Drug-metabolizing enzymes in the kidney play a significant role in the metabolism of many xenobiotics. Kidneys have many metabolic responsibilities including the hydroxylation of fatty acids and their derivatives, which is one of the functions of the CYP4A and CYP4B enzymes expressed in renal microsomes. There is increasing interest in renal metabolism due to the significant role that kidneys play in the metabolism of drugs and the fact that the kidney is a major site of drug-induced toxicity.

To assess the stability of human and animal renal subcellular fractions, pooled samples were subjected to several freeze-thaw cycles, and analyzed for lauric acid 12-hydroxylation, umbelliferone glucuronidation and NADPH-cytochrome c reduction. As shown below, renal samples prepared by XenoTech’s subcellular fractionation protocol can be repeatedly frozen and thawed at least ten times without significant loss of CYP4A activity. Similar results were obtained for umbelliferone glucuronidation and NADPH-cytochrome c reduction.

Packaging

Micro­somes
Concentration: 10 mg/mL
Suspension buffer: 250 mM sucrose

S9 fraction
Concentration: 5 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

Characterization Provided

XenoTech’s kidney subcellular fractions are characterized for the following activities:

• NADPH-cytochrome c reductase
• Lauric Acid 12-hydroxylation
• 4-Methylumbelliferone glucuronidation

Related Products

XenoTech also offers subcellular fractions of renal tissue from the following toxicologically significant species:

• Human
• Cynomolgus monkey
• IGS Sprague-Dawley rat

For other extrahepatic or subcellular fractions, please contact us to discuss our wide selection of Custom Products.  We have extensive experience preparing a variety of unique research products (from human and laboratory animal sources) for a growing list of customers.

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