Golden Syrian Hamster Subcellular Fractions

View Products

PackagingCharacterization ProvidedProduct Details

Liver microsomes con­tain cytochromes P450, flavin-monooxygenases, carboxyl­esterases, epoxide hydrolase, UDP-glu­cur­ono­syl­transferases and other drug-metabolizing enzymes, and are intended for use in in vitro studies of xenobiotic metabolism.

The S9 fraction is a mixture of microsomes and cytosol. Accordingly, it contains a wide variety of Phase 1 and Phase 2 enzymes including P450 enzymes, flavin-mono­oxygenases, carboxyl­esterases, epoxide hydrolase, UDP-glucuronosyl­transferases, sulfotransferases, methyl­transferases, acetyl­trans­ferases, gluta­thione S-trans­fer­ases and other drug-metabolizing enzymes.

Liver cytosol contains many xenobiotic metabolizing enzymes including carboxylesterases, soluble epoxide hydrolases, alcohol dehydrogenases, aldehydes oxidases, xanthine oxidases, diamine oxidases, sulfotransferases, glutathione S-transferases, etc.

Packaging

Micro­somes
Concentration: 20 mg/mL
Suspension buffer: 250 mM sucrose

S9 fraction
Concentration: 20 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

Cytosol
Concentration: 10 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

Characterization Provided

The following lot-specific product information is included with each order of non-human liver microsomes.

• Specific content of cytochrome P450
• Specific content of cytochrome b5
• NADPH-cytochrome c reductase activity
• 7-Ethoxycoumarin O-dealkylation
• Animal Information and Husbandry

Product Details

NOMENCLATURE Lak:LVG(SYR)BR ORIGIN Three members of a litter captured in Syria in 1930 were retained in captivity. It is the progeny of these animals that were first imported to the United States in 1938. Descended from two original colonies acquired by Lakeview in 1949 and 1951. Closed outbred colony since 1951. COAT COLOR Medium tan.

Products