Human Kidney Subcellular Fractions

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Kidneys have many metabolic responsibilities including the hydroxylation of fatty acids and their derivatives. There is increasing interest in renal metabolism due to the fact that the kidney can play a significant role in the metabolism of many xenobiotics and is a major site of drug-induced toxicity.

Human kidneys express relatively high levels of CYP4A and CYP4B enzymes, phase II drug-metabolizing enzymes including UGT1A6, UGT1A9/10, UGT2B7, SULT1C2 and DT-diaphorase (NQO1) and the efflux transporter P-glycoprotein (MDR1).

To assess the stability of human and animal renal subcellular fractions, pooled samples were subjected to several freeze-thaw cycles, and analyzed for lauric acid 12-hydroxylation, umbelliferone glucuronidation and NADPH-cytochrome c reduction. As shown below, renal samples prepared by XenoTech’s subcellular fractionation protocol can be repeatedly frozen and thawed at least ten times without significant loss of CYP4A activity. Similar results were obtained for umbelliferone glucuronidation and NADPH-cytochrome c reduction.

Packaging

Micro­somes
Concentration: 10 mg/mL
Suspension buffer: 250 mM sucrose

S9 fraction
Concentration: 5 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

Characterization Provided

• NADPH-cytochrome c reductase
• Lauric Acid 12-hydroxylation
• 4-Methylumbelliferone glucuronidation

Related Products

Kidney subcellular fractions from other species:

• Cynomolgus Monkey
• Beagle dog
• IGS Sprague-Dawley rat

For other extrahepatic fractions, contact us and ask about our Custom Products option.  We have experience preparing a variety of unique research products (from human and laboratory animal sources) for a growing list of customers.

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