Human Lung Subcellular Fractions

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PackagingCharacterization ProvidedSafety, Handling, and StorageRelated Products

As the primary site for the entrance of airborne agents, and as the recipient of all cardiac output, the lungs may provide both first-pass and systemic metabolism of xenobiotics through several enzymatic pathways including both cytochrome P450 and phase II enzymes.

Packaging

Microsomes
Concentration: 10 mg/mL
Suspension buffer: 250 mM sucrose

S9 fraction
Concentration: 5 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

Characterization Provided

• NADPH-cytochrome c reductase
• 7-Ethoxyresorufin O-dealkylation
• 4-Methylumbelliferone glucuronidation

Safety, Handling, and Storage

Informed Consent Statement

A single organization regulates and oversees the use of human tissue intended for transplantation in the United States, namely the United Network for Organ Sharing (UNOS). Organ donors may elect to have their organs used either for transplantation only, or for transplantation or research. Thus, the donor (or the donor's family) has the right to prevent the use of the donor's organs for research. Regardless of the use of donated organs, no compensation is given to the donor’s family; any such compensation is illegal in the United States. In those cases where donors (or family members) elect to withhold organs from research uses, any organs that cannot be transplanted are discarded.

XenoTech receives hepatic, renal, intestinal, pulmonary, and other human tissue from various regional organ procurement organizations (OPOs) that obtain organs approved for research use. Regulations in the United States require that, regardless whether the organ is intended for transplantation or research purposes, the organ donor's identity be treated as highly confidential information. Organ procurement organizations maintain the informed consent records from each donor, and our Standard Operating Procedure requires that XenoTech personnel confirm the existence of informed consent for research purposes, prior to transport of organs to XenoTech. This procedure is intended to ensure that XenoTech manufactures human-derived products only when informed consent has been granted for research use of those specific organs. XenoTech does not, and, in consideration of confidentiality, cannot obtain the informed consent records from these organizations.

All human tissue accepted by XenoTech has been tested for the possible presence of various infectious diseases, and XenoTech does not accept human-derived material unless the donor has tested negative (non-reactive) for RPR, HIVAb, HTLVAb, HBsAg and HCVAb. All human tissue is also tested for CMVAb.  However, due to the widespread (nearly ubiquitous) appearance of CMV in the population, and its relative insignificance as an infectious agent, tissues from donors reactive for CMVAb are accepted.  The serology status of each donor is typically determined by ELISA by the organ procurement hospital.

XenoTech does not deal directly with - nor does it make any direct payments to - the surgeons who procure organs or the medical institutions where they work.

Storage

Subcellular fractions should be stored in an ultra-low freezer (-70°C or colder).

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• Cynomolgus Monkey
• Beagle dog
• IGS Sprague-Dawley rat

Caution

In the case of human tissue, XenoTech accepts only non-transplantable tissue from donors who test negative for HIV 1 and 2, HTLV, and Hepatitis B and C. However, as a precaution, all human-de­rived samples should be regarded as a potential bio­hazard and should be stored, handled and discarded accordingly. Cellular and subcellular fractions are intended for in vitro use only.

Products

Product #	Name	Gender	Amount
H0610.P(NS)	Pooled Human Lung Microsomes, Non-smokers	Mixed	0.5 mL at 10 mg/mL
H0610.P(S)	Pooled Human Lung Microsomes, Smokers	Mixed	0.5 mL at 10 mg/mL
H0610.PS9(NS)	Pooled Human Lung S9, Non-smokers	Mixed	1.0 mL at 5 mg/mL
H0610.PS9(S)	Pooled Human Lung S9, Smokers	Mixed	1.0 mL at 5 mg/mL