Rhesus Monkey Subcellular Fractions

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PackagingCharacterization ProvidedSafety, Handling, and Storage

Liver microsomes con­tain cytochromes P450, flavin-monooxygenases, carboxyl­esterases, epoxide hydrolase, UDP-glu­cur­ono­syl­transferases and other drug-metabolizing enzymes and are intended for in vitro studies of xenobiotic metabolism.

The S9 fraction is a mixture of microsomes and cytosol. Accordingly, it contains a wide variety of Phase 1 and Phase 2 enzymes including P450 enzymes, flavin-mono­oxygenases, carboxyl­esterases, epoxide hydrolase, UDP-glucuronosyl­transferases, sulfotransferases, methyl­transferases, acetyl­trans­ferases, gluta­thione S-trans­fer­ases and other drug-metabolizing enzymes.

Liver cytosol contains many xenobiotic metabolizing enzymes including carboxylesterases, soluble epoxide hydrolases, alcohol dehydrogenases, aldehydes oxidases, xanthine oxidases, diamine oxidases, sulfotransferases, glutathione S-transferases, etc.

Packaging

Microsomes
Concentration: 20 mg/mL
Suspension buffer: 250 mM sucrose

S9 fraction
Concentration: 20 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

Cytosol
Concentration: 10 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

Characterization Provided

The following lot-specific product information is included with each order of non-human liver microsomes.

• Specific content of cytochrome P450
• Specific content of cytochrome b5
• NADPH-cytochrome c reductase activity
• 7-Ethoxycoumarin O-dealkylation
• Animal Information and Husbandry

Safety, Handling, and Storage

Policy Statement Concerning the Safety of Non-human Primate-derived Material

XenoTech manufactures products derived from non-human primate tissue for the purpose of xenobiotic research. All tissue used to manufacture these products is obtained from reliable sources within the United States, and is obtained from disease-free (asymptomatic) animals under the care of a veterinarian.

In accordance with federal (USDA) regulations, all non-human primates entering the United States are placed in quarantine for a period of thirty days. If an animal is infected with Ebola virus, it would die during the quarantine period, and hence, would not reside in a primate colony in the United States. For this reason, Ebola virus testing is not a common procedure in the United States. Animals are tested for Simian Herpes B virus (SBV), Simian Retrovirus (SRV), and Simian Immunodeficiency virus (SIV). All non-human primate tissue utilized at XenoTech is obtained from animals which have tested seronegative for these viruses.

Although animals are quarantined and tested for certain infectious diseases, it is not scientifically possible to guarantee that non-human primate-derived material is free of all infectious agents and poses absolutely no health risk. Therefore, we strongly caution all researchers who use non-human primate-derived material to treat such material as a potential biohazard, and to observe all national, regional, and local regulations governing the handling of such material. Even if non-human primate-derived material has been tested for the presence of specific infectious agents by highly sensitive techniques, such as RT-PCR, and found to test negative, the material should still be treated as a potential biohazard.

Please note:  XenoTech complies with all regulations promulgated by the Convention on International Trade in Endangered Species (CITES). Accordingly, special permits and arrangements must be made to ship all primate products internationally.  Customers in Europe and Japan should contact our distributor nearest you for information on pricing and availability.

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