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Calendar of Events
| Date | Event Name |
|---|---|
| May 23, 2012 | Webinar: PBPK modeling |
Wistar Rat Subcellular Fractions
PackagingCharacterization ProvidedProduct Details
View microsome lot information and characterization data here!
View S9 lot information and characterization data here!
Liver microsomes contain cytochromes P450, flavin-monooxygenases, carboxylesterases, epoxide hydrolase, UDP-glucuronosyltransferases and other drug-metabolizing enzymes, and are intended for use in in vitro studies of xenobiotic metabolism.
The S9 fraction is a mixture of microsomes and cytosol. Accordingly, it contains a wide variety of Phase 1 and Phase 2 enzymes including P450 enzymes, flavin-monooxygenases, carboxylesterases, epoxide hydrolase, UDP-glucuronosyltransferases, sulfotransferases, methyltransferases, acetyltransferases, glutathione S-transferases and other drug-metabolizing enzymes.
Liver cytosol contains many xenobiotic metabolizing enzymes including carboxylesterases, soluble epoxide hydrolases, alcohol dehydrogenases, aldehydes oxidases, xanthine oxidases, diamine oxidases, sulfotransferases, glutathione S-transferases, etc.
Packaging
Microsomes
Concentration: 20 mg/mL
Suspension buffer: 250 mM sucrose
S9 fraction
Concentration: 20 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA
Cytosol
Concentration: 10 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA
Characterization Provided
The following lot-specific product information is included with each order of non-human liver microsomes.
- Specific content of cytochrome P450
- Specific content of cytochrome b5
- NADPH-cytochrome c reductase activity
- 7-Ethoxycoumarin O-dealkylation
- Animal Information and Husbandry
Product Details
NOMENCLATURE Crl:(WI)BR ORIGIN To Scientific Products Farm, Ltd. [predecessor of Charles River United Kingdom (CRUK)] in 1947 from Wistar Institute. To CRL in 1975 from CRUK. Caesarean rederived in 1975 to form the nucleus of the current Wistar rat colonies. This particular colony was selected because of a low incidence of hydronephrosis. COAT COLOR Albino.
Products
| Product # | Name | Gender | Amount |
| R3000 | Pooled Wistar Rat Liver Microsomes | Male | 0.5 mL at 20 mg/mL |
| R3000.C | Pooled Wistar Rat Liver Cytosol | Male | 1.0 mL at 10 mg/mL |
| R3000.S9 | Pooled Wistar Rat Liver S9 Fraction | Male | 1.0 mL at 20 mg/mL |
| R3500 | Pooled Wistar Rat Liver Microsomes | Female | 0.5 mL at 20 mg/mL |
| R3500.C | Pooled Wistar Rat Liver Cytosol | Female | 1.0 mL at 10 mg/mL |
| R3500.S9 | Pooled Wistar Rat Liver S9 Fraction | Female | 1.0 mL at 20 mg/mL |
