PREDIVEZ Vesicular Transport Kits

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PREDIVEZ Fluorescent Vesicular Kits
The PREDIVEZ™ Vesicular Transport kit is a simple and powerful tool to investigate drug transporter interactions. It provides information on any interaction between the ABC transporter and the drug candidate that would affect the transport of the fluorescent reporter substrate.

 

 

 

 

 

 

These kits deliver many advantages when compared to conventional products.

Features include:

• Sensitive and quick flourescent measurements
• Larger dynamic range / signal-to-background ratio compared to the radioactive Vesicular transport assay
• Flexible data analysis
• Reproducible results

Data can be presented in transport values (with the help of the calibration curve, included), or in percentages (see Figures below).

 

 

 

 

 

 

 

 

 

 

 

 

All reagents and solutions are in one easy-to-use kit, each kit is accompanied by a CD that includes the assay protocol, excel data evaluation sheets, MSDS and reference curves with known interacters.

PREDIVEZ Radiolabled Vesicular Kits

SOLVO’s new P-gp PREDIVEZ™ kit: a breakthrough in P-gp interaction assessment, measures drug-transporter interaction in vesicular transport membrane vesicles expressing either human
P-gp (MDR1) or rat Mdr1b.

P-gp (ABCB1, MDR1) is the most commonly studied ABC transporter with important roles not only in tumor multidrug resistance but in the ADMETox properties of drugs and nutrients. As most of the substrates of P-gp are highly membrane permeable, the challenge was to develop a vesicular transport assay with a low permeability reporter substrate. SOLVO's new P-gp PREDIVEZ™ Kit operates with a low permeability cationic radio-labeled substrate that is transported by both MDR1 and ratMdr1b transporters. The typical dynamic range (cpms in ATP+/cpms in ATP-) is ~ 10 in the P-gp and ~ 3 in the ratMdr1b assay.

Human P-gp PREDIVEZ™ kit works with human membrane vesicles while the rat Mdr1b PREDIVEZ™kit contains insect membrane preparation expressing the rodent ABC transporter.

This revolutionary PREDIVEZ™ kit allows a faster and more cost effective investigation of the drug candidate and P-gp interaction than lower throughput cellular assays, such as Caco-2, MDCK-MDR1 or LLC-PK MDR1 cell-based systems.

SOLVO’s new Pg-p vesicular transport assay operates with a low permeability, cationic radiolabeled substrate (N-methyl-quinidine) that is transported by both human MDR1 and rat Mdr1b transporters.

Both kits are available in three sizes designed to measure interaction of 3, 6 or 9 compounds. Negative control membranes and positive control reference materials are included.

The price of the kit includes the cost of the radiolabeled reporter substrate, which is shipped separately. The labeled reporter compound comes in a single size package, sufficient for testing 9 compounds. PREDIVEZ™ VT kits are also available without the labeled reporter compound.

Each kit is accompanied by a CD that includes the assay protocol, Excel spreadsheet with data processing template, MSDS and reference curves with known interactors.

For More information on the new P-gp PREDIVEZ™ kit, please visit SOLVO's website.

Products

Product # 3 Compound	Product # 6 Compound	Product # 9 Compound	Name	Transporter
SBPV001	SBPV002	SBPV003	SB MRP2 PREDIVEZ VT	MRP2
SBPV004	SBPV005	SBPV006	SB rat Mrp2 PREDIVEZ VT	rat Mrp2
SBPV007	SBPV008	SBPV009	SB MRP3 PREDIVEZ VT	MRP3
SBPV010	SBPV011	SBPV012	SB MRP5 PREDIVEZ VT	MRP5
SBPV019	SBPV020	SBPV021	SB BCRP PREDIVEZ VT	BCRP
SBPV022	SBPV023	SBPV024	SB P-gp (MDR1) PREDIVEZ VT-Without radiolabeled substrate	MDR1
SBPV022+	SBPV023+	SBPV024+	SB P-gp (MDR1) PREDIVEZ VT- With radiolabeled substrate	MDR1
SBPV025	SBPV026	SBPV027	SB rat Mdr1b PREDIVEZ VT-Without radiolabeled substrate	Mdr1
SBPV025+	SBPV026+	SBPV027+	SB rat Mdr1b PREDIVEZ VT- With radiolabeled substrate	Mdr1
SBPV028	SBPV029	SBPV030	SB MRP1 PREDIVEZ VT	MRP1